With Codon-Usage robot, user can analyze the codon usage bias. Codon-Usage robot can be found at firstname.lastname@example.org.
Before you start analyzing codon usage bias using this robot, make sure that you have a sequence selected in the workspace.
After selecting the sequence into workspace, user can specify open reading frame (ORF) parameters. Also user can specify the organism for which the codon usage in the sequence is to be analyzed. Additionally user can specify the start codon for the ORF, otherwise default start codon (ATG) is used.
Codon Usage Finding
So based on the parameters entered by the user, Codon-Usage robot finds all the open reading frames in all the frames first and then highlights all the codons that are not acceptable (which are very less frequent) for the selected organism. The output of the Codon-Usage robot is similar to the the following image. It displays the original sequence, followed by all the open reading frames that are found in a reading frame. Multiple frames found one after the other are also displayed and then all the unacceptable codons are highlighted.