How to design a primer
If you want to design a set of primers please select the sequence from the workspace that you want to amplify using PCR. If you have more than one or no sequence at all selected the Primer Designer will not work.
Now click the “Design Primer” Button in the Primer Designer’s menu. You will see the menu as shown in the image above. In this menu you can select the desired melting temperature for the primers. If you want to you can also specify a start and endpoint in the target sequence. By default the robot will design a primer for the whole sequence.
Once you’re done with the settings click “send”.
The robot will now design the primers and display them like this: